Anatomy in Action
Video demonstration of some basic methods.
Courtesy of the Brain Observatory, University of California at San Diego.
1.1 Perfusion Stain:
Methylene Blue & Fixation of Brain in situ
1.1.1 Solutions for
Methylene Blue Perfusion Stain
Paraformaldehyde Solution
Methylene Blue Solution
Molybdate Solution
Saline/Heparin/Papaverine Solution
1.1.2
Conventional Transcardiac Perfusion
Instruments and Materials
Procedure
1.1.3
Transcarotid Perfusion
Instruments and Materials
Procedure
1.1.4
Alternative Techniques Tested
Photograph Blockface of Fresh Unperfused Brain
Photograph Blockface of Frozen Unperfused Brain
Omit Molybdate from Methylene Blue Protocol
Omit Papaverine from Methylene Blue Protocol
Perfusion Stain by Cresyl Violet
Perfusion Stain by Potassium Dichromate